other:Article Title: Autophagy collaborates with apoptosis pathways to control oligodendrocyte number.
Article Snippet: Primary antibodies used include: rabbit anti-TFEB (Bthyl Laboratories, 1:2000, Cat# A303-673A), mouse anti-b-actin (Santa Cruz Biotechnology, 1:500, Cat#sc-47778), rat anti-MBP (Abcam, 1:500, Cat#ab7349), rabbit anti-Beclin1 (Cell Signaling Technology, 1:1000, Cat#3495S), rabbit anti-ATG5 (Cell Signaling Technology, 1:1000, Cat#12994S), rabbit anti-ATG7 (Cell Signaling Technology, 1:1000, Cat#8558S), rabbit anti-PUMA (Cell Signaling Technology, 1:1000, Cat#24633S), rat anti-LAMP2 (Abcam, 1:1000, Cat#ab13524), rabbit anti-p62 (Cell Signaling Technology, 1:1000, Cat# 5114S), rabbit anti-Bak (Cell Signaling Technology, 1:1000, Cat#12105T), rabbit anti-Bax (Cell Signaling Technology, 1:1000, Cat#2772T), rabbit anti-XIAP (Cell Signaling Technology, 1:200, Cat#14334S), mouse anti-Bcl2 (Santa Cruz Biotechnology, 1:200, Cat# sc-7382), mouse anti-WIPI2 (Bio-Rad, 1:1000, Cat#mca5780GA), mouse anti-LC3 (MBL, 1:500, Cat#M186-3), rabbit anti-c-Jun (Cell Signaling Technology, 1:1000, Cat#9165T), rabbit anti-phospho-c-Jun (Ser73) (Cell Signaling Technology, 1:1000, Cat#3270T), and mouse anti-GAPDH (Santa Cruz Biotechnology, 1:500, Cat#sc-32233).
Article Title: Oncogenic mutations convert MET from a pro-apoptotic tumor suppressor to an oncogenic driver
Article Snippet: The primary antibodies and dilutions used were: mouse anti-MET (Cell Signaling Technology, Danvers, MA, #3148; 1:1000), rabbit anti-phospho-MET (Tyr1234/1235) (Cell Signaling Technology #3126; 1:1000), rabbit anti-phospho-MET (Tyr1003) (clone 13D11; Cell Signaling Technology #3135; 1:1000), rabbit anti-MET (clone SP44; Abcam, Cambridge, MA, #ab227637; 1:1000), rabbit anti-c-CBL (Cell Signaling Technology #2747; 1:1000), mouse anti-AKT (Cell Signaling Technology, #2920; 1:2000), rabbit anti-phospho-AKT (Ser473) (Cell Signaling Technology #4060; 1:2000), mouse anti-ERK2 (clone D2; Santa Cruz Biotechnology, Dallas, TX, #sc-1647; 1:1000), mouse anti-phospho-ERK1/2 (Thr202/Tyr204) (clone E10; Cell Signaling Technology, #9106; 1:1000), rabbit anti-PARP1 (clone H250; Santa Cruz Biotechnology #sc-7150; 1:1000), rabbit anti-cleaved caspase-3 (Asp175) (Cell Signaling Technology #9661; 1:1000), rabbit anti-Bak (Cell Signaling Technology #3814, 1:500), rabbit anti-Bax (Cell Signaling Technology #2774, 1:500), rabbit anti-Bcl-xL (clone 54H6, Cell Signaling Technology #2764, 1:1000), rabbit anti-Bid (Cell Signaling Technology #2002, 1:1000), rabbit anti-Bim (Cell Signaling Technology #2819, 1:500), rabbit anti-Mcl-1 (clone D35A5, Cell Signaling Technology #5453, 1:1000), mouse anti-Noxa (Calbiochem, San Diego, CA, #114C307, 1:250), rabbit anti-Puma (Cell Signaling Technology #4976, 1:500), mouse anti-actine (clone C4, Millipore #MAB1501, 1:4000), mouse anti-α-tubulin (clone B-5-1-2; Santa Cruz Biotechnology #sc-23948; 1:1000), mouse anti-GAPDH (clone 6C5; Santa Cruz Biotechnology #sc-32233).
Article Title: Targeting CHD1L suppresses prostate cancer progression via the FOXO3-PUMA axis
Article Snippet: The antibodies used in this study were against the following: rabbit anti-CHD1L(1:1000; #32923-1-AP; Proteintech), rabbit anti-Cyclin B1 (1:1000; #4138; CST), rabbit anti-Cyclin E1 (1:1000; #20808; CST), rabbit anti-Cdk1 (1:1000; #ET1607-51; HUABIO), rabbit anti-Cdk2 (1:1000; #ET1602-6; HUABIO), rabbit anti-BAX (1:1000; #2772; CST), rabbit anti-BCL-2 (1:1000; #3498; CST), rabbit anti-FOXO3 (1:1000; #2497; CST), rabbit anti-PUMA (1:1000; #98672; CST), rabbit anti-Tubulin (1:2000; #AF001; Beyotime).
Article Title: Targeting CHD1L suppresses prostate cancer progression via the FOXO3-PUMA axis.
Article Snippet: The antibodies used in this study were against the following: rabbit anti193 CHD1L(1:1000; #32923-1-AP; Proteintech), rabbit anti-Cyclin B1 (1:1000; #4138; 194 CST), rabbit anti-Cyclin E1 (1:1000; #20808; CST), rabbit anti-Cdk1 (1:1000; 195 #ET1607-51; HUABIO), rabbit anti-Cdk2 (1:1000; #ET1602-6; HUABIO), rabbit 196 anti-BAX (1:1000; #2772; CST), rabbit anti-BCL-2 (1:1000; #3498; CST), rabbit 197 anti-FOXO3 (1:1000; #2497; CST), rabbit anti-PUMA (1:1000; #98672; CST), 198 rabbit anti-Tubulin (1:2000; #AF001; Beyotime).
Article Title: Autophagy collaborates with apoptosis pathways to control oligodendrocyte number
Article Snippet: Primary antibodies used include: rabbit anti-TFEB (Bthyl Laboratories, 1:2000, Cat# A303–673A), mouse anti-β-actin (Santa Cruz Biotechnology, 1:500, Cat#sc-47778), rat anti-MBP (Abcam, 1:500, Cat#ab7349), rabbit anti-Beclin1 (Cell Signaling Technology, 1:1000, Cat#3495S), rabbit anti-ATG5 (Cell Signaling Technology, 1:1000, Cat#12994S), rabbit anti-ATG7 (Cell Signaling Technology, 1:1000, Cat#8558S), rabbit anti-PUMA (Cell Signaling Technology, 1:1000, Cat#24633S), rat anti-LAMP2 (Abcam, 1:1000, Cat#ab13524), rabbit anti-p62 (Cell Signaling Technology, 1:1000, Cat# 5114S), rabbit anti-Bak (Cell Signaling Technology, 1:1000, Cat#12105T), rabbit anti-Bax (Cell Signaling Technology, 1:1000, Cat#2772T), rabbit anti-XIAP (Cell Signaling Technology, 1:200, Cat#14334S), mouse anti-Bcl2 (Santa Cruz Biotechnology, 1:200, Cat# sc-7382), mouse anti-WIPI2 (Bio-Rad, 1:1000, Cat#mca5780GA), mouse anti-LC3 (MBL, 1:500, Cat#M186–3), rabbit anti- c -Jun (Cell Signaling Technology, 1:1000, Cat#9165T), rabbit anti-phospho- c -Jun (Ser73) (Cell Signaling Technology, 1:1000, Cat#3270T), and mouse anti-GAPDH (Santa Cruz Biotechnology, 1:500, Cat#sc-32233).
Western Blot:Article Title: Obatoclax enhances TRAIL-induced apoptosis in non-small-cell-lung-cancer cells by increasing TRAIL receptor expression, priming for mitochondrial apoptosis and reducing XIAP levels
Article Snippet: The broad-spectrum caspase inhibitor zVADfmk was purchased from Promega, Madison, USA. .. For Western blotting, we followed the recommendations of manufacturers and the following primary antibodies were used: mouse monoclonal anti-caspase 8 (1C12, Cell Signaling Technology, Danvers, MA, USA, 1:1,000), rabbit anti-caspase 9 (Cell Signaling Technology, Danvers, MA, USA, 1:1,000), anti-cleaved-caspase 9 (D330) (Cell Signaling Technology, Danvers, MA, USA, 1:1,000), rabbit anti-caspase 3, rabbit anti-cleaved caspase 3 (Asp175) (Thermo Fisher Scientific, Waltham, MA, USA, 1:1,000), rabbit anti-PARP (Cell Signaling Technology, Danvers, MA, USA, 1:500), rabbit antiBcl-2 (Cell Signaling Technology, Danvers, MA, USA, 1:500), rabbit anti-Bcl-xL (Cell Signaling Technology, Danvers, MA, USA, 1:1,000), rabbit anti-Mcl-1 (Cell Signaling Technology, Danvers, MA, USA, 1:500), rabbit anti-cytochrome c (Cell Signaling Technology, Danvers, MA, USA, 1:500), rabbit anti-COX IV (Cell Signaling Technology, Danvers, MA, USA, 1:500), rabbit anti-Puma (Cell Signaling Technology, Danvers, MA, USA, 1:500), rabbit-anti-BID (Cell Signaling Technology, Danvers, MA, USA, 1:1,000), rabbit anti-Bax (Cell Signaling Technology Danvers, USA), goat anti-Bak (G-23)(Santa Cruz Biotechnology, Heidelberg, Germany). .. 1:1,000), mouse anti-β-actin (Sigma-Aldrich Chemicals, Zwijndrecht, The Netherlands, 1:10,000), rabbit anti-TRAIL receptor 2 (Cell Signaling Technology, Danvers, MA, USA, 1:1,000), mouse anti-NOXA (114C307) (Merck KGaA, Darmstadt, Germany, 1:500), mouse anti-XIAP clone 2F1 (MBL International, Woburn, USA, 1:1,000), goat anti-DR4 (C20) (Santa Cruz Biotechnology, Heidelberg, Germany, 1:5,000), and mouse anti-GAPDH (sc-32233) (Santa Cruz Biotechnology, Heidelberg, Germany, 1:10,000).
Incubation:Article Title: Meningeal lymphatic vessel dysfunction exacerbates brain injury in CVST mice via endoplasmic reticulum and oxidative stress pathways
Article Snippet: .. Sections were then blocked with 5% goat serum (Zsbio, Cat. No. ZLI-9022, China) for 30 minutes at room temperature, followed by incubation with the following primary antibodies at 37°C for 1 hour: rabbit anti-CHOP (1:200, 15204-1-AP, Proteintech); rabbit anti-ATF4 (1:200, ET1612-7, Huabio); rabbit anti-PUMA (1:100, 98672T, CST); rabbit anti-Caspase-12 (1:200, A22864, Clona); rabbit anti-CD68(1:200 97778S, CST);anti- Bcl-2(1:400 68103-1-lg, Proteintech); rabbit anti-Bax(1:500 ab32503, abcam);rabbit anti-NLRP3(1:100 ab270449 abcam);anti- IL-1β(1:100 HA601002 Huabio). ..
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